HIV-2 Viral Load - Guide to Services

Requisition Forms

PCR, Serology and Viral Load Requisition for HIV-1/2 Testing
NHRL-HRS-LABF038-8 NLHRS HIV 1-2 Requisition PCR, Serology & VL.pdf

GUIDELINES FOR SUBMITTING SPECIMENS TO NLHRS
NHRL-HRS-LABF027-11 GUIDELINES FOR SUBMITTING SPECIMENS TO NLHRS.pdf

Reference Details

Description:

HIV-2 Viral Load.

Test Category:

Molecular Quantification

Pathogen:

Human immunodeficiency virus (HIV)

Laboratory:

National Laboratory for HIV Reference Services

Illnesses and Diseases:

Acquired immunodeficiency syndrome (AIDS)

Specimen:

HIV-2 Viral Load: Plasma; minimum 2 mL. Contact NLHRS for specimens other than those listed above.

Collection Method:

See specimen above.

Specimen Processing, Storage and Shipping:

Freeze specimens at -70°C and ship frozen on dry ice.

Transportation of Dangerous Goods:

Shipping of specimens shall be done by a TDG certified individual in accordance with TDG regulations. For additional information regarding classification of specimens for the purposes of shipping, consult either Part 2 Appendix 3 of the TDG Regulations or section 3.6.2 of the IATA Dangerous Goods Regulations as applicable.

Patient Criteria:

Please provide risk factors.

Accompanying Documentation:

NLHRS HIV 1/2 Requisition: PCR, Serology & Viral Load

Comments:

See attached NLHRS Submission Guidelines.

Methods and Interpretation of Results:

Testing is performed, in whole or in part, using a lab-developed test which has not been fully validated/verified due to a lack of well-characterized panel.

Varies for each test method, contact NLHRS for further information.

Turnaround Time:

Varies for each test method, contact NLHRS for further information.

Contact:

Phone #: (204) 789-6522

Fax: (204) 318-2221

Email: nlhrs-lnsrv@phac-aspc.gc.ca

References:

Standard Council of Canada. SCC accredits first medical laboratory under new national program. 2008. http://www.scc.ca/en/news-events/news/2008/scc-accredits-first-medical-laboratory-under-new-national-program
S.Lavoie, D. Caswell, M.J. Gill, K. Kadkhoda, C.L Charlton, P.N. Levett, T.Hatchette, R. Garceau, J. Maregmen, T. Mazzulli, R. Needle, K. Kadivar, J. Kim. Heterophilic interference in specimens yielding false-reactive results on the Abbott 4th generation ARCHITECT HIV Ag/Ab Combo Assay. Journal of Clinical Virology. 2018;104: 23-28
Malloch L, Kadivar K, Putz J, Levett PN, Tang J, Hatchette TF, Kadkhoda K, Ng D,Ho J, Kim J. Comparative Evaluation of the Bio-Rad Geenius HIV-1/2 Confirmatory Assay and the Bio-Rad Multispot HIV-1/2 Rapid Test as an Alternative Differentiation Assay for CLSI M53 Algorithm-I. Journal of Clinical Virology. 2013;58, Supplement 1(0):e85-e91. http://www.sciencedirect.com/science/article/pii/S1386653213003326
Kadivar K, Malloch L, Adonsou-Hoyi Y, Ng D, Lavoie S, Pulido K, Kim J. Would CLSI M53-A Have Helped in the Diagnosis of HIV in Canada? Results of the Performance of Canadian Laboratories Participating in a Recent NLHRS Proficiency Testing Panel Containing HIV-1 Antigen Positive (Antibody Negative) and HIV-2 Samples. Journal of Clinical Virology. 2013;58(1):303-305. http://www.sciencedirect.com/science/article/pii/S1386653213001303

Fact Sheets:

Pathogen Safety Data Sheets: Infectious Substances – Human immunodeficiency virus (HIV)

Guidelines:

Biosafety Directive for Human Immunodeficiency Virus (HIV) and Human T-cell Lymphotropic Virus Type 1 (HTLV-1)

Human Immunodeficiency Virus - HIV Screening and Testing Guide

Canadian Guidelines on Sexually Transmitted and Blood-Borne Infections

National case definition: Acquired immune deficiency syndrome (AIDS)

HIV and AIDS: For health professionals

National case definition: Human immunodeficiency virus (HIV)

Related Information:

HIV and AIDS